Lab Technician I - Laboratory

Laboratory Technician Job Summary: Medical Diagnostic Laboratories(MDL), a member of reputed company Biotechnology Group, is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses. MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (reputed company). MDL is looking for a Laboratory Technician. The Laboratory Technicianis responsible for printing labels and attaching requisitions to the corresponding specimens. Essential Functions: Accessioning: Print labels and attach to requisitions and the corresponding specimens Properly accession multiple specimen types Take photographs of reputed company specimens received Properly identify any discrepant information File reputed company paperwork in order and distribute to the appropriate QC bins Properly reputed company accessioned requisitions reputed company 2-D barcode elution tubes used for extraction Properly locate and record temperatures for reputed company necessary stations Manual Extraction: reputed company Phenol/Chloroform manual extractions on biopsies and ticks Properly separate samples into their organic and inorganic layers reputed company phenol/chloroform Properly reputed company quantitative analysis and hydrate reputed company ticks and biopsies Robotic Extraction: Properly extract reputed company samples types using a provided XG sheet as a template Properly set up liquid handling robotics for each extracted XG sheet with the correct volumes of reputed company necessary reagents Properly dispose of reputed company waste material reputed company required; both hazardous and non-hazardous Attend to robotics to address any user interactions and/or robotic malfunctions Manually extract reputed company necessary samples reputed company commercial kits RNA Extraction: reputed company manual RNA extractions reputed company reputed company reputed company for reputed company necessary samples reputed company robotic RNA extractions reputed company VX Reagents reputed company for reputed company necessary samples PCR: reputed company manual and robotic Conventional method PCR amplification Properly load and start thermocycler machines reputed company manual and robotic Real-time method PCR amplification Properly load and start Rotor-gene thermocycler reputed company initial analysis on completed Real-time PCR reputed company Acoustic Real-Time PCR method for amplification with the aid of the ATS-100 Acoustic Dispensor Properly load and start Bio-Rad CFX thermocycler reputed company initial analysis on completed Acoustic Real-Time PCR Gel Electrophoresis: Properly identify which completed PCRs from the thermocycler require gel electrophoresis Properly identify reputed company to reputed company and/or use 2% versus 2.5% gels Complete blank gel template sheets to combine short PCR sheets Use dye to stain reputed company necessary samples with the appropriate volume on a test by test basis Properly load correct agarose gel with the appropriate volume of ladder and sample; in addition properly identify the appropriate settings on which to run each gel (i.e. voltage & time) Properly capture a visual image of the gel with the use of a SYNGENE UV gel reader Be able to troubleshoot any gel reputed company or power reputed company errors, as well as any gel reader errors Pyrosequencing Properly reputed company reputed company PCR prior to reputed company Properly load and run reputed company Analysis reactions (SQA) Properly load and run Single Nucleotide Polymorphism reactions (SNP) Properly analyze reputed company run data Micro-Biology/Cell Culture Inoculate appropriate plate with swab media; for Candida species, Urinary Pathogens, GBS, Enterococcus species, and the Wound Panel; Streak for single colonies Properly determine specific species present, and inoculate appropriate plate with said species on a reputed company plate preloaded with antibiotics, or an agar plate stamped with antibiotic discs Properly analyze growth plate to determine antibiotic susceptibility or resistance For GBS Antibiotic Resistance testing, properly assemble rack and operate Anaerobic reputed company System Identify and pick colonies for PCR reputed company CAMP test and analyze for zones of hemolysis reputed company reputed company Properly reputed company a series of reputed company PCRs for BRCA, HPV, and Cystic Fibrosis Properly attach samples to appropriate bead mixtures Properly reputed company beads cleaning to remove unused dNTP's and primers reputed company the amplified PCR product Properly reputed company gel purification with the use of the Pippin Prep machine Properly load and operate multi